Bioethanol Production Optimization from KOH-Pretreated Bombax ceiba Using Saccharomyces cerevisiae through Response Surface Methodology

The present study was based on the production of bioethanol from alkali-pretreated seed pods Bombax ceiba. Pretreatment is necessary to properly utilize for via fermentation. This process assures accessibility cellulase cellulose found in seedpods by removing lignin. Untreated, KOH-pretreated, and KOH-steam-pretreated substrates were characterized morphological, thermal, chemical changes scanning electron microscopy (SEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR). Hydrolysis biomass performed using both commercial indigenous cellulase. Two different fermentation approaches used, i.e., separate hydrolysis (SHF) simultaneous saccharification (SSF). Findings show that maximum (58.6% after 24 h) highest ethanol titer (57.34 g/L 96 observed KOH-steam-treated substrate SSF. SSF further optimized physical nutritional parameters one factor at a time (OFAT) central composite design (CCD). optimum (72.0 g/L) 0.25 yeast extract, 0.1 K2HPO4, (NH4)2SO4, 0.09 MgSO4, 8% substrate, 40 IU/g cellulase, 1% Saccharomyces cerevisiae inoculum, pH 5.